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Literature summary extracted from

  • Li, P.; Li, K.; Li, X.; Zhao, F.; Wang, R.; Wang, J.
    Improving enzyme activity of glucosamine-6-phosphate synthase by semi-rational design strategy and computer analysis (2020), Biotechnol. Lett., 42, 2319-2332 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.6.1.16 gene glmS, screening of mutant library, recombinant expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) Bacillus subtilis

Protein Variants

EC Number Protein Variants Comment Organism
2.6.1.16 A594G site-directed mutagenesis, the mutant shows unaltered activity (5 U/l) compared to wild-type (5 U/l) Bacillus subtilis
2.6.1.16 K595H site-directed mutagenesis, the mutant shows unaltered activity (5 U/l) compared to wild-type (5 U/l) Bacillus subtilis
2.6.1.16 L593S site-directed mutagenesis, the mutant shows highly increased activity (48 U/l) compared to wild-type (5 U/l) Bacillus subtilis
2.6.1.16 additional information improvement of enzyme BsGlms activity through computer simulation and site saturation mutagenesis, method, overview Bacillus subtilis
2.6.1.16 S596F site-directed mutagenesis, the mutant shows slightly increased activity (7 U/l) compared to wild-type (5 U/l) Bacillus subtilis
2.6.1.16 V597R site-directed mutagenesis, the mutant shows slightly increased activity (8 U/l) compared to wild-type (5 U/l) Bacillus subtilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.16 additional information
-
 additional information binding energy calculations of wild-type and mutant enzymes Bacillus subtilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.1.16 L-glutamine + D-fructose 6-phosphate Bacillus subtilis
-
 L-glutamate + D-glucosamine 6-phosphate
-
 ?
2.6.1.16 L-glutamine + D-fructose 6-phosphate Bacillus subtilis 168
-
 L-glutamate + D-glucosamine 6-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.16 Bacillus subtilis P0CI73
-
-
2.6.1.16 Bacillus subtilis 168 P0CI73
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.16 L-glutamine + D-fructose 6-phosphate
-
 Bacillus subtilis L-glutamate + D-glucosamine 6-phosphate
-
 ?
2.6.1.16 L-glutamine + D-fructose 6-phosphate
-
 Bacillus subtilis 168 L-glutamate + D-glucosamine 6-phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.6.1.16 BsglmS
-
 Bacillus subtilis
2.6.1.16 GlmS
-
 Bacillus subtilis
2.6.1.16 glucosamine-6-phosphate synthase
-
 Bacillus subtilis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.6.1.16 37
-
 assay at Bacillus subtilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.16 7.5
-
 assay at Bacillus subtilis

General Information

EC Number General Information Comment Organism
2.6.1.16 additional information mutation of residue Ser593 enhances the flexibility of the protein, which ultimately leads to increased enzyme activity, molecular dynamics simulation, homology modeling, and protein-ligand docking, overview Bacillus subtilis